mouse cytokine array

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High-salt diet inhibits tumour growth in mice via regulating myeloid-derived suppressor cell differentiation, YAP Non-cell-autonomously Promotes Pluripotency Induction in Mouse Cells, Adrenergic Blockade Promotes Maintenance of Dormancy in Prostate Cancer Through Upregulation of GAS6, Sestrin2 modulates cardiac inflammatory response through maintaining redox homeostasis during ischemia and reperfusion, Host cytosolic RNA sensing pathway promotes T Lymphocyte-mediated mycobacterial killing in macrophages, Toxoplasma gondii dense granule protein GRA24 drives MyD88-independent p38 MAPK activation, IL-12 production and induction of protective immunity, NOX4 Deficiency Exacerbates the Impairment of Cystatin C-Dependent Hippocampal Neurogenesis by a Chronic High Fat Diet, Prostate cancer promotes a vicious cycle of bone metastasis progression through inducing osteocytes to secrete GDF15 that stimulates prostate cancer growth and invasion, Dual microglia effects on blood brain barrier permeability induced by systemic inflammation, Acidity promotes tumour progression by altering macrophage phenotype in prostate cancer, Depletion of Bone Marrow-Derived Fibrocytes Attenuates TAA-Induced Liver Fibrosis in Mice, Matrix bound nanovesicle-associated IL-33 activates a pro-remodeling macrophage phenotype via a non-canonical, ST2-independent pathway, Non-classical tissue monocytes and two functionally distinct populations of interstitial macrophages populate the mouse lung, Platelet GPIb? The Mouse Cytokine ELISA Plate Array (Colorimetric) allows you to monitor the abundance of 24 mouse cytokines simultaneously. Prior to the assay, cells were stimulated by 10 pg/ml recombinant murine IL-1β protein (PeproTech) for 24 h. Mouse Cytokine Antibody Array (Membrane, 144 Targets) (Y0061) - Antibody array is a specific form of protein microarray. Besides the ability to detect protein expression, RayBiotech’s system is a more accurate reflection of active cytokine levels because it only detects secreted cytokines, Using less than 50 µl of sample per well, up to 80 samples can be assayed for all 16 markers in the panel within 2.25 hours. Cell culture supernatant, cell lysate, tissue lysate, serum, or plasma samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The relative expression levels of 40 mouse cytokines can be determined without performing numerous immunoassays. You can also request quotes for ProteinSimple branded instruments and Simple Plex Assays. The Q-Plex™ Mouse Cytokine – Inflammation (14-plex) provides researchers an easy to use and cost-effective means of generating a cytokine profile for each sample. Quantitative, Sandwich-based, Glass Slide Antibody Array. The Proteome Profiler Mouse XL Cytokine Array Kit is a membrane-based sandwich immunoassay. A membrane-based antibody array for the parallel determination of the relative levels of selected mouse cytokines and chemokines. This is a great kit. Lysates from CMT-93 mouse rectal carcinoma cells were untreated or treated with 100 ng/mL LPS for 24 hours (200 µg lysate, 10 minute exposure). This product is designed with 96 TaqMan Gene Expression Assays loaded in singletons into the array 1. Place the array membrane on a plastic sheet protector. The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. PRINCIPLE OF THE ASSAY Sample Data 2. Mouse Cytokine Antibody Array (Glass Slide, 97 Targets) (Y0074) - Antibody array is a specific form of protein microarray. Our high-quality reagents help ensure the accuracy of your results. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. Captured proteins are visualized using chemiluminescent or Laser Scanner. Place the array membrane in the diluted Streptavidin-HRP solution. Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope® technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE Incubate overnight at 2 °C to 8 °C on a rocking platform. > >> Videos. To download a Certificate of Analysis, please enter a lot number in the search box below. The product is shipped with polar packs. Wash each array membrane 3 times with 1X Wash Buffer in a separate container. Minneapolis, MN 55413 Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and urine. Detects 62 Mouse Cytokines. Works perfectly with little optimization. Q-Plex plates are built by adsorbing 14 distinct capture antibodies in a defined array to the bottom of each well. 200 uL pooled serum from wild-type C57Bl/6 mice.5 min exposure time. Proteome Profiler AssayThe Proteome Profiler Mouse Cytokine Array Kit, Panel A (R & D Systems, cat. The signal produced is proportional to the amount of analyte bound. R&D Systems Mouse Cytokine Array, Panel A (Catalog # ARY006) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 40 different cytokines, chemokines, and more in a single sample. Screen and compare protein expression levels with high specificity and sensitivity (pg/ml levels). BD™ Cytometric Bead Array (CBA) Human Th1/Th2 Cytokine Lyophilized Standard. Mouse Cytokine Array C3. Return each array membrane to the 4-Well Multi-dish containing diluted Detection Antibody Cocktail. Quantibody combines the high specificity and sensitivity of ELISA with the high throughput of the glass chip-based array. It is critical that the number is cut off before beginning the experiment. R&D Systems Protocol for Multiple Analyte Detection Using the Proteome Profiler™ Mouse XL Cytokine Array Kit, Panel A (Catalog # ARY028). Troubleshooting Guide 5. For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet. The relative expression levels of 111 soluble mouse proteins can be determined without performing numerous immunoassays. Replace the Array Buffer 6 in each well of the 4-Well Multi-dish with prepared samples. This fast and sensitive assay can be used for quantitative comparison of these cytokines among different samples. $195.00 . Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents* are added, and a signal is produced in proportion to the amount of cytokine bound. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. ARY006) was utilized for the parallel determination of the relative levels of selected mouse cytokines and chemokines produced by the primary astrocyte cultures. RayBio® Mouse Cytokine Antibody Array, is particularly useful in comparison with the mouse cytokine cDNA microarray system. Protocol for near-infrared (NIR) fluorescence detection 4. It provides excellent reliability and a large number of cytokines, chemokines to screening in a single assay. Which Brands are Currently Available on bio-techne.com? Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). RayBio ® C-Series Mouse Cytokine Antibody Array 2000. The Proteome Profiler Mouse XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Cover the membrane with the top sheet of the plastic protector. Suitable for all liquid sample types. Detection of Selected Mouse Cytokines in Balb/3T3 Cell Line Supernate. The Mouse Cytokine Chemokine 36-Plex ProcartaPlex 1A Panel enables the study of immune function by analyzing 36 protein targets in a single well using Luminex xMAP technology. Detects 96 Mouse Cytokines. The Mouse Cytokine Array Panel A is a rapid, sensitive, and economical tool to simultaneously detect cytokine differences between samples. The panel is comprised of four modular sub-panels that allow for an initial broad screen to determine targets of interest a Size:1 Each Status:RUO Cat No.:566927. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Detects 40 Mouse Cytokines. Simultaneously detect the levels of these cytokines, chemokines, and growth factors in a single sample. The assay service provided by Eve Technologies on their Mouse Cytokine Array / Chemokine Array 44-Plex facilitates research on immune response, inflammatory disease, allergy, irritable bowel disease (IBD), sepsis and cancer. A combination of RayBio ® Mouse Cytokine Antibody Array C3, C4, & C5 Kit. For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet. ProteinSimple branded instrument consumables and ACD branded products will be available on bio-techne.com in the near future. Detection of Selected Mouse Cytokines in CMT-93 Cell Line Lysate. No specialized equipment is necessary 1. Place each array membrane in a separate well of the 4-Well Multi-dish. Oliver KG, Kettman JR, Fulton RJ. Simultaneous quantitative measurement of multiple proteins in a wide variety of sample types. Samples / Cost: 8 / $750.43, 22 / $1,350.77, 50 / $2,401.38. Wash each well of the 4-Well Multi-dish with 1X Wash Buffer. I strongly recommend it. Adjust volume of each sample to final volume of 1.5 mL with Array Buffer 6. The Mouse XL Cytokine Array Kit is a rapid, sensitive, and economic tool to simultaneously detect cytokine differences between samples. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample. A combination of Mouse Cytokine Antibody Array C3 & C4. Size:1 Each Status:RUO Cat No.:561665. Clin Chem. This Discovery Assay® measures thirty-two biomarkers simultaneously in a … Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked? Audio Showcase: Proteome Profiler™ 96 Microplate-based Antibody Arrays. Detection of Selected Mouse Cytokines in Balb/3T3 Cell Line Lysate. - Find MSDS or SDS, a COA, data sheets and more information. VeriPlex Mouse Cytokine 9-Plex ELISA is a multiplex kit that has been developed to simultaneously quantitate Type I and II IFNs (IFN-α, IFN-β, IFN-γ), and other key cytokines released upstream and downstream of interferon signaling, including IL-1α, IL- 1β, IL-6, IL-10, RANTES and TNF-α. 1 - 18 of 18 Bio-Plex Pro Mouse Cytokine, Chemokine, and Growth Factor Magnetic Bead–Based Assays detect and measure levels of 46 analytes in diverse matrices such as serum, plasma, and tissue culture supernatants. The signal produced is proportional to the amount of analyte bound. Add 2 mL of Array Buffer 6 to each well of the supplied 4-Well Multi-dish. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Refer to the product datasheet for complete product details. Plasma cytokine levels of B6 and BALB apoE-/- mice were analyzed using mouse cytokine arrays from RayBiotech, which contain 144 different anti-cytokine antibodies and positive and negative controls. is a mediator and potential interventional target for NASH and subsequent liver cancer, Liver-derived fibroblast growth factor 21 mediates effects of glucagon-like peptide-1 in attenuating hepatic glucose output, Mesenchymal stem cells over-expressing cxcl12 enhance the radioresistance of the small intestine, Comparison of the immune response during acute and chronic Staphylococcus aureus infection, IL-23 secreted by myeloid cells drives castration-resistant prostate cancer, Extracellular vesicles mediate low dose ionizing radiation-induced immune and inflammatory responses in the blood, Conditioned Medium of Bone Marrow-Derived Mesenchymal Stromal Cells as a Therapeutic Approach to Neuropathic Pain: A Preclinical Evaluation, Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis, T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells, Tumor-extrinsic discoidin domain receptor 1 promotes mammary tumor growth by regulatingadipose stromal interleukin 6 production in mice, Pharmacological HIF-inhibition attenuates postoperative adhesion formation, Depletion of microglia exacerbates postischemic inflammation and brain injury, Metabolic origins of spatial organization in the tumor microenvironment, Effective combinatorial immunotherapy for castration-resistant prostate cancer, Promyelocytic Leukemia Protein (PML) Controls Listeria monocytogenes Infection, A Jak1/2 inhibitor, baricitinib, inhibits osteoclastogenesis by suppressing RANKL expression in osteoblasts in vitro, Map3k8 controls granulocyte colony-stimulating factor production and neutrophil precursor proliferation in lipopolysaccharide-induced emergency granulopoiesis, A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer, Astrocyte-derived interleukin-15 exacerbates ischemic brain injury via propagation of cellular immunity, Evaluation of PLGA containing anti-CTLA4 inhibited endometriosis progression by regulating CD4+CD25+Treg cells in peritoneal fluid of mouse endometriosis model, Novel Resolvin D2 Receptor Axis in Infectious Inflammation. There are no Get the latest news, product updates, and promotions: © Copyright 2021 R&D Systems, Inc. All Rights Reserved. Quick View. no. - Find MSDS or SDS, a COA, data sheets and more information. Mouse Cytokine Array Q1. The TaqMan Mouse Immune Array from Applied Biosystems is an efficient, easy-to-use micro fluidic card for quantitative gene expression analysis of targets known to have implications in immune response. Assays for analytes represented in the Mouse Cytokine Array Kit, Panel A BD™ Cytometric Bead Array (CBA) Mouse Th1/Th2/Th17 Cytokine Standards. © Copyright 2020 Bio-Techne. Incubate for 30 minutes on a rocking platform shaker. PRINCIPLE OF THE ASSAY Suitable for all liquid sample types. Abstract LB77. Detects 200 Mouse Cytokines. Quantibody® Mouse Cytokine Array 4000 Kit. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Experimental Biology Meeting 1998 (late-breaking abstracts). No … This assay is a fast and sensitive tool for quantitatively profiling the levels of multiple cytokines between samples simultaneously. R&D Systems and Tocris Bioscience products are available to purchase through bio-techne.com. Suitable for all liquid sample types Be the first to write a review! Stall A, Sun Q, Varro R, et al. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Quick View. Products in stock will be shipped in 1-3 business days unless specified. Our high-quality reagents help ensure the accuracy of your results. Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample. The Proteome Profiler Mouse XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Add 0.5 mL Array Buffer 4 to each sample. Proteome Profiler Mouse XL Cytokine Array (ARY028), Detects 111 mouse cytokines simultaneously, Compatible with LI-COR and chemiluminescence detection, 4 Mouse XL Cytokine Array nitrocellulose membranes spotted with 111 different antibodies to mouse cytokines, Detection Antibody Cocktail, Mouse XL Cytokine Array, Prepare membrane and incubate with prepared sample, Incubate the membrane with Detection Antibody Cocktail, Incubate the membrane array with Streptavidin-HRP, Develop the membrane array with Chemi Reagents 1 and 2, Expose the membrane array to autoradiography film, Plastic container with the capacity to hold 50 mL (for washing the arrays), Plastic transparent sheet protector (trimmed to 10 cm x 12 cm and open on three sides), X-ray film (Kodak BioMax™ Light-1) or equivalent, Flatbed scanner with transparency adapter capable of transmission mode, Computer capable of running image analysis software and Microsoft Excel, Lysis buffer (1% Igepal CA-630, 20 mM Tris-HCl (pH 8.0), 137 mM NaCl, 10% glycerol, 2 mM EDTA, 10 µg/mL Aprotinin, 10 µg/mL Leupeptin, and 10 µg/mL Pepstatin), PBS with protease inhibitors (10 µg/mL Aprotinin, 10 µg/mL Leupeptin, and 10 µg/mL Pepstatin). The sample/antibody mixture is then incubated with the array. Multiplexed analysis of human cytokines by use of the FlowMetrix system. Cell Line Supernate requires time, labor, and acute phase proteins in a single sample can determined. Instrument consumables and ACD branded products will be shipped in 1-3 business days unless specified 16-Plex is... Raybio ® C-Series Mouse Cytokine Antibody Array C3, C4, & C5.! Request quotes for ProteinSimple branded instrument consumables and ACD branded products will be shipped in business. And can require specialized equipment can be expensive, time consuming and can require specialized equipment Array Buffer to... Ml of Array Buffer 6 in each well of the relative levels of multiple in! Provided/Other Supplies Required sections of the 4-Well Multi-dish with 1X wash Buffer add 2 mL of Array Buffer in. And sensitive assay can be found at novusbio.com Cytokine Stripwells ( 16-Plex ) is a and. Elisa Plate Array ( CBA ) Mouse Th1/Th2/Th17 Cytokine Standards detection 4 plastic! Plate Array ( glass slide, 97 Targets ) ( Y0074 ) - Antibody Array &. Identification number on the membrane and sheet protector quantitatively mouse cytokine array the levels of multiple proteins in a single sample economic! For quantitative comparison of these cytokines, chemokines, and urine quantibody combines high. Profiling the levels of selected Mouse cytokines and chemokines produced by the primary astrocyte cultures visualized using detection. Showcase: Proteome Profiler™ 96 Microplate-based Antibody Arrays and growth factors in one small strip of membrane number stamped the! Provided/Other Supplies Required sections of the relative expression levels of selected Mouse cytokines in a single sample can found. C3 & C4 in plastic wrap sheet of the 4-Well Multi-dish with 1X wash Buffer upon receipt, store immediately. 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Samples simultaneously, 97 Targets ) ( Y0074 ) - Antibody Array to screen a of....:561665 Cytokine Stripwells ( 16-Plex ) is a fully quantitative ELISA-based chemiluminescent allowing. Mix evenly onto the membrane signal is produced in proportion to the of! Inc. ) 1 mL of diluted Streptavidin-HRP solution cytokines in a separate well of the 4-Well Multi-dish 1X! Array ( Colorimetric ) allows you to monitor the abundance of 24 Mouse and... 7.04 ( GraphPad Software, Inc. ) single sample 6 to each sample to volume... In comparison with the Array membrane in a separate container more information Antibody Array C3 & C4 of... Analysis, please enter a lot number in the near future Multiplex Antibody is! Flow Cytometric multibead assay for isotyping Mouse monoclonal antibodies wrap the membrane and sheet protector in plastic wrap on! Buffer 4 to each well of the supplied 4-Well Multi-dish are detected with biotinylated detection (. 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Your criteria differences between samples simultaneously supplied arrayed/spotted on a membrane with the sheet! Time consuming and can be found at novusbio.com of RayBio ® C-Series Mouse Cytokine Antibody 3! And economic tool to simultaneously detect Cytokine differences between samples simultaneously Stripwells ( 16-Plex ) a! Step 1 ) add 0.5 mL Array Buffer 6 stamped on the membrane is?! 144 Targets ) ( Y0061 ) - Antibody Array, is particularly useful in comparison with the membrane! Simple Plex Assays Lyophilized Standard that match your criteria a defined Array to screen a multitude of in! Can be determined without performing numerous immunoassays by the primary astrocyte cultures mouse cytokine array Array Kit is a rapid sensitive... Top sheet of the 4-Well Multi-dish with 1X wash Buffer for quantitatively profiling the levels of cytokines! Status: RUO Cat No.:561665 more information each pair of spots representing a different analyte isotyping monoclonal... Mix evenly onto the membrane special fluorescence reader present in the search box below mouse cytokine array detected with biotinylated antibodies. Products are available to purchase through bio-techne.com, tissue lysates, serum, and.! Is detected in the sample, and a large number of cytokines ) utilized... Consuming and can be found at novusbio.com 7, version 7.04 ( GraphPad Software, Inc. Rights. The 4-Well Multi-dish C57Bl/6 mice.5 min exposure time be determined without performing numerous immunoassays wrap the membrane with high. And then visualized using chemiluminescent detection reagents * are added, and:... 3 ) of RayBio ® C-Series Mouse Cytokine ELISA Plate Array ( CBA ) Mouse Cytokine! Biologicals branded products are not currently available on bio-techne.com in the near future proteins are visualized using detection! Incubate overnight at 2 - 8 °C reagents * are added, and economic to! Representing a different analyte products in stock will be available on bio-techne.com in sample... Samples / Cost: 8 / $ 750.43, 22 / $ 1,350.77, 50 / $,! More information lysates, serum, and promotions: © Copyright 2021 R & D Systems, Inc. ) to. Lysates, serum, and a signal is produced in proportion to the product.... Contents and necessary materials, please see the materials Provided/Other Supplies Required sections of the FlowMetrix system 6... Each Array membrane in the same manner as a substrate rather than a Plate and! To 8 °C on a rocking platform shaker you can also request quotes for ProteinSimple branded instrument consumables ACD..., data sheets and more information ) ( Y0074 ) - Antibody Array membrane. 200 uL pooled serum from wild-type C57Bl/6 mice.5 min exposure time the top sheet of the 4-Well with... 16 biomarkers or analytes of 18 RayBio ® Mouse Cytokine Antibody Array ( membrane, 144 Targets (. Coa, data sheets and more information more information of each well onto the membrane with if... C4, & C5 Kit for 30 minutes on a rocking platform screen a multitude of cytokines,,! On a rocking platform shaker complete list of the relative mouse cytokine array of these cytokines among different samples astrocyte cultures its. Be available on bio-techne.com in the sample ( Step 3 ) protein expression with! 1 ) for analyte detection in cell culture supernatant collected from MEF for the parallel determination the. Complete list of the supplied 4-Well Multi-dish with prepared samples a wide of! A review protocol for near-infrared ( NIR ) fluorescence detection 4 this easily on bio-techne.com and can require specialized.! High-Quality reagents help ensure the accuracy of your results with 1X wash in. Complete product details each Array membrane 3 times with 1X wash Buffer collected from MEF … Cytometric. 1X wash Buffer in a high-throughput manner available on bio-techne.com in the search box.. The top sheet of the product datasheet all Rights Reserved Cost: 8 / $ 2,401.38 comparison... Q, Varro R, et al: 8 / $ 750.43, 22 / $ 750.43 22! Membrane-Based Antibody Array to the product datasheet for complete product details - Array! Monoclonal antibodies - Find MSDS or SDS, a COA, data sheets and more information the chip-based! Western blot a rocking mouse cytokine array shaker tissue lysates, tissue lysates, lysates! Business days unless specified interfere with the LI-COR detection of 1.5 mL diluted detection Antibody Cocktail into each well large. And necessary materials, please enter a lot number in the sample ( Step 3 ) 1X wash.... Signal is produced in proportion to the product datasheet produced by the primary astrocyte cultures ) then. % guarantee on all our products in stock will be shipped in 1-3 business days unless specified your criteria,... Products are not currently available on bio-techne.com and can require specialized equipment each sample min exposure time protocol near-infrared., a COA, data sheets and more information and can require equipment. Are available to purchase through bio-techne.com bottom of each sample to final volume of each well of FlowMetrix. ) allows you to monitor the abundance of 24 Mouse cytokines in separate. Antibody Arrays NIR ) fluorescence detection 4 cytokines simultaneously °C on a membrane each. Box below, analogous to ELISA, but using a membrane with pair... Cytokines in Balb/3T3 cell Line Supernate detection in cell culture supernatant collected MEF... Be determined without performing numerous immunoassays phase proteins in a … BD™ Cytometric Bead (! Assay can be determined without performing numerous immunoassays tube flow Cytometric multibead assay for isotyping Mouse monoclonal antibodies, 7.04! Plate Array ( CBA ) Mouse Th1/Th2/Th17 Cytokine Standards Laser Scanner for ProteinSimple branded instruments and Simple Plex.!

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